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. 1987 Nov;84(21):7735-8.
doi: 10.1073/pnas.84.21.7735.

Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues

F Thiebaut  1 , T Tsuruo, H Hamada, M M Gottesman, I Pastan, M C Willingham
Affiliations

Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues

F Thiebaut et al. Proc Natl Acad Sci U S A. 1987 Nov.

Abstract

Monoclonal antibody MRK16 was used to determine the location of P-glycoprotein, the product of the multidrug-resistance gene (MDR1), in normal human tissues. The protein was found to be concentrated in a small number of specific sites. Most tissues examined revealed very little P-glycoprotein. However, certain cell types in liver, pancreas, kidney, colon, and jejunum showed specific localization of P-glycoprotein. In liver, P-glycoprotein was found exclusively on the biliary canalicular front of hepatocytes and on the apical surface of epithelial cells in small biliary ductules. In pancreas, P-glycoprotein was found on the apical surface of the epithelial cells of small ductules but not larger pancreatic ducts. In kidney, P-glycoprotein was found concentrated on the apical surface of epithelial cells of the proximal tubules. Colon and jejunum both showed high levels of P-glycoprotein on the apical surfaces of superficial columnar epithelial cells. Adrenal gland showed high levels of P-glycoprotein diffusely distributed on the surface of cells in both the cortex and medulla. These results suggest that the protein has a role in the normal secretion of metabolites and certain anti-cancer drugs into bile, urine, and directly into the lumen of the gastrointestinal tract.

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