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Genetic map of the primocane-fruiting and thornless traits of tetraploid blackberry

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Abstract

Blackberry primocane fruiting, fruiting on first-year canes, has the potential to expand blackberry production both seasonally and geographically. The incorporation of the primocane-fruiting trait into cultivars with desirable horticultural attributes is challenging due to its recessive nature and tetrasomic inheritance. Molecular marker-assisted selection has high potential to facilitate incorporation, because breeders already use morphological marker-assisted selection of seedlings without marginal cotyledonary hairs to identify progeny that will be thornless when mature. The development of a genetic linkage map with these two traits is the first step to utilizing molecular markers in breeding for thornless primocane-fruiting blackberry cultivars. A full-sib family segregating for thornlessness and primocane fruiting, from a cross between ‘APF-12’ and ‘Arapaho’, was used to construct the first genetic map of tetraploid blackberry. Segregation patterns of several dominant markers and the two phenotypic traits fit those expected uniquely with tetrasomic inheritance (e.g., 5:1, 11:1 and 35:1). Some loci showed significant double reduction frequencies, but genotypes that could have originated only from double reduction were not found. The map consists of seven linkage groups (LG) in each parent, consistent with the basic number of chromosomes (2n = 4x = 28). Naming of LG1-LG6 followed that of the recently revised system for raspberry using SSR markers in common between blackberry and raspberry, and LG7 was tentatively defined by default. The loci controlling primocane fruiting and thornlessness were not linked to each other; thornless/thorny, the S Locus, was mapped on LG4, and the primocane-/floricane-fruiting locus, named in this work the F Locus, on LG7.

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Acknowledgments

P. Castro is grateful to Andalusian Institute of Agricultural Research and Training (IFAPA) and European Social Fund (ESF) for a postdoctoral fellowship and the hosting institution of the fellowship, USDA-ARS-GIFVL. This project was partially funded by USDA-ARS Project 1245-21220-185-00 and by the University of Arkansas fruit breeding program. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture or any of the other agencies involved in this research.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The experiments of this study comply with the current laws of USA.

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Authors and Affiliations

  1. IFAPA, Centro ‘Alameda del Obispo’, Mejora y Biotecnologia, Avenida Menendez Pidal, s/n, 14080, Córdoba, Spain

    P. Castro

  2. Mississippi State University, Southern Branch Experiment Station, 810 Highway 26 West, P. O. BOX 193, Poplarville, MS, 39470, USA

    E. T. Stafne

  3. Department of Horticulture, University of Arkansas, 316 Plant Science, Fayetteville, AR, 72701, USA

    J. R. Clark

  4. USDA-ARS, Genetic Improvement of Fruits and Vegetables Laboratory, 10300 Baltimore Ave., Building 010A, Beltsville, MD, 20705, USA

    K. S. Lewers

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  1. P. Castro
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Corresponding author

Correspondence to K. S. Lewers.

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Communicated by D. A. Lightfoot.

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Castro, P., Stafne, E.T., Clark, J.R. et al. Genetic map of the primocane-fruiting and thornless traits of tetraploid blackberry. Theor Appl Genet 126, 2521–2532 (2013). https://doi.org/10.1007/s00122-013-2152-3

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