Use of locus-specific AFLP markers to construct a high-density molecular map in barley
- PMID: 24710875
- DOI: 10.1007/s001220050752
Use of locus-specific AFLP markers to construct a high-density molecular map in barley
Abstract
By using 25 primer combinations, 563 AFLP markers segregating in a recombinant inbred population (103 lines, F9) derived from L94/Vada were generated. The 38 AFLP markers in common to the existing AFLP/RFLP combined Proctor/Nudinka map, one STS marker, and four phenotypic markers with known map positions, were used to assign present AFLP linkage groups to barley chromosomes. The constructed high-density molecular map contains 561 AFLP markers, three morphological markers, one disease resistance gene and one STS marker, and covers a 1062-cM genetic distance, corresponding to an average of one marker per 1.9 cM. However, extremely uneven distributions of AFLP markers and strong clustering of markers around the centromere were identified in the present AFLP map. Around the centromeric region, 289 markers cover a genetic distance of 155 cM, corresponding to one marker per 0.5 cM; on the distal parts, 906 cM were covered by 277 markers, corresponding to one marker per 3.3 cM. Three gaps larger than 20 cM still exist on chromosomes 1, 3 and 5. A skeletal map with a uniform distribution of markers can be extracted from the high-density map, and can be applied to detect and map loci underlying quantitative traits. However, the application of this map is restricted to barley species since hardly any marker in common to a closely related Triticum species could be identified.
Similar articles
-
A high-density molecular map for ryegrass (Lolium perenne) using AFLP markers.Theor Appl Genet. 1999 Aug;99(3-4):445-52. doi: 10.1007/s001220051256. Theor Appl Genet. 1999. PMID: 22665177
-
Development of AFLP markers in barley.Mol Gen Genet. 1997 Apr 16;254(3):330-6. doi: 10.1007/s004380050423. Mol Gen Genet. 1997. PMID: 9150268
-
Combined mapping of AFLP and RFLP markers in barley.Mol Gen Genet. 1995 Nov 1;249(1):65-73. doi: 10.1007/BF00290237. Mol Gen Genet. 1995. PMID: 8552035
-
Enrichment of a papaya high-density genetic map with AFLP markers.Genome. 2009 Aug;52(8):716-25. doi: 10.1139/g09-043. Genome. 2009. PMID: 19767901
-
A molecular genetic map and electrophoretic karyotype of the plant pathogenic fungus Cochliobolus sativus.Mol Plant Microbe Interact. 2002 May;15(5):481-92. doi: 10.1094/MPMI.2002.15.5.481. Mol Plant Microbe Interact. 2002. PMID: 12036279
Cited by
-
The reference genetic linkage map for the multinational Brassica rapa genome sequencing project.Theor Appl Genet. 2007 Oct;115(6):777-92. doi: 10.1007/s00122-007-0608-z. Epub 2007 Jul 24. Theor Appl Genet. 2007. PMID: 17646962
-
QTLs Controlling Physiological and Morphological Traits of Barley (Hordeum vulgare L.) Seedlings under Salinity, Drought, and Normal Conditions.BioTech (Basel). 2022 Jul 15;11(3):26. doi: 10.3390/biotech11030026. BioTech (Basel). 2022. PMID: 35892931 Free PMC article.
-
An integrated high-density linkage map of soybean with RFLP, SSR, STS, and AFLP markers using A single F2 population.DNA Res. 2007 Dec 31;14(6):257-69. doi: 10.1093/dnares/dsm027. Epub 2008 Jan 11. DNA Res. 2007. PMID: 18192280 Free PMC article.
-
An AFLP-based genome-wide mapping strategy.Theor Appl Genet. 2004 Jan;108(2):321-7. doi: 10.1007/s00122-003-1427-5. Epub 2003 Sep 16. Theor Appl Genet. 2004. PMID: 13679979
-
Two loci on chromosome 5H determine low-temperature tolerance in a 'Nure' (winter) x 'Tremois' (spring) barley map.Theor Appl Genet. 2004 Feb;108(4):670-80. doi: 10.1007/s00122-003-1468-9. Epub 2003 Oct 24. Theor Appl Genet. 2004. PMID: 14576984
LinkOut - more resources
Full Text Sources
Miscellaneous
