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Haemophilus influenzae Protocols

  • Book
  • © 2003

Overview

Editors:
  1. Mark A. Herbert

    1. Department of Pediatrics, John Radcliffe Hospital, Oxford, UK

  2. Derek W. Hood

    1. Department of Pediatrics, John Radcliffe Hospital, Oxford, UK

  3. E. Richard Moxon

    1. Department of Pediatrics, John Radcliffe Hospital, Oxford, UK

Part of the book series: Methods in Molecular Medicine (MIMM, volume 71)

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About this book

As the second leading bacterial cause of community-acquired pneumonia, H. influenzae accounts for many thousands of deaths per year worldwide, and is a prime candidate for the identification of targets that can lead to the development of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of this significant bacterium. Described with step-by-step instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. The antibody techniques presented include IVIAT, ELISA, and opsonophagocytosis assays. Also included are reviews of the pathogenesis of NTHi and Hib, methods for working with the organism, culture and storage conditions, molecular diagnosis, and systems for exploring pathogenesis in both in vitro and animal models.
Comprehensive and highly practical, Haemophilus influenzae Protocols offers a unique collection of powerful molecular methods that illuminate not only how the bacterium causes disease, but also how best to develop much-needed new vaccines and antibiotics against it.

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Pathogenesis of Disease Due to Nontypeable Haemophilus influenzae

    • Gail G. Hardy, Simone M. Tudor, Joseph W. St. Geme III
    Pages 1-28

  3. The Pathogenesis of Disease Due to Type b Haemophilus influenzae

    • Ruth Aubrey, Christoph Tang
    Pages 29-50

  4. General Methods for Culturing Haemophilus influenzae

    • Grant Poje, Rosemary J. Redfield
    Pages 51-56

  5. Transformation of Haemophilus influenzae

    • Grant Poje, Rosemary J. Redfield
    Pages 57-70

  6. Diagnosis of Infection

    • Alex van Belkum, Loek van Alphen
    Pages 71-92

  7. Characterization of Plasmids

    • Ioanna D. Dimopoulou, Mohd-Zain Zaini, Derrick W. Crook
    Pages 93-99

  8. Protein Characterization by Two-Dimensional Gel Electrophoresis

    • Phillip Cash, J. Simon Kroll
    Pages 101-118

  9. Monitoring Gene Expression Using DNA Arrays

    • Tahir R. Ali, Ming-Shi Li, Paul R. Langford
    Pages 119-134

  10. Gene Expression Technology

    • Xavier de Bolle, Christopher D. Bayliss
    Pages 135-146

  11. The Genome Sequence of Haemophilus influenzae

    • Derek W. Hood
    Pages 147-160

  12. Structural Profiling of Short-Chain Lipopolysaccharides from Haemophilus influenzae

    • Elke K. H. Schweda, James C. Richards
    Pages 161-183

  13. Mutagenesis of H. influenzae

    • Mark A. Herbert
    Pages 185-209

  14. Transposon Tn 10

    • Stefan Schlör, Gabriele Kemmer, Joachim Reidl
    Pages 211-224

  15. In Vivo Expression of Bacterial Genes During Human Infections

    • Martin Handfield, Trevor Seifert, Jeffrey D. Hillman
    Pages 225-242

  16. ELISA

    • Joyce S. Plested, Philip A. Coull, Margaret Anne J. Gidney
    Pages 243-261

  17. Opsonophagocytosis Assay Using Flow-Cytometry

    • Joyce S. Plested, Philip A. Coull
    Pages 263-275

  18. In Vitro Models of Infection I—Human Respiratory Tissue Organ Culture

    • Andrew M. Middleton, Paula Keig, Robert Wilson
    Pages 277-295

  19. In Vitro Models of Infection II—Human Umbilical Vein Endothelial Cells (HUVECs) System

    • Mumtaz Virji, Darryl J. Hill
    Pages 297-314

  20. Animal Models

    • Mumtaz Virji, Darryl J. Hill
    Pages 315-322
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Reviews

"This excellent book is recommended for investigators involved in laboratory research on H. influenzae from the beginning student to the experienced investigator. The book contains abundant, detailed protocols and practical tips that will be useful for all investigators." - Clinical Infectious Diseases

"...an excellent source book and an inspiring reading." - International Journal of Medical Microbiology

"In addition to detailed information provided in the individual chapters, the book is also valuable by providing an extensive list of 841 references....there are not many microbiological books of such high quality. The authors present highly specialized information in a comprehensible and friendly way." -Folia Microbioligica

Editors and Affiliations

  • Department of Pediatrics, John Radcliffe Hospital, Oxford, UK

    Mark A. Herbert, Derek W. Hood, E. Richard Moxon

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Bibliographic Information

  • Book Title: Haemophilus influenzae Protocols

  • Editors: Mark A. Herbert, Derek W. Hood, E. Richard Moxon

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592593216

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 2003

  • Hardcover ISBN: 978-0-89603-928-5Published: 25 September 2002

  • Softcover ISBN: 978-1-61737-255-1Published: 09 November 2010

  • eBook ISBN: 978-1-59259-321-7Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XI, 333

  • Topics: Infectious Diseases

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