Identification and distribution of a two-pore domain potassium channel in the CNS of Aplysia californica
- PMID: 15306118
- DOI: 10.1016/j.molbrainres.2004.05.007
Identification and distribution of a two-pore domain potassium channel in the CNS of Aplysia californica
Abstract
A cDNA encoding a potassium channel of the two-pore domain family (K2p) of leak channels was cloned from the CNS of the marine opisthobranch Aplysia californica. This is the first sequence of the K2p family identified in molluscs and has been named AcK2p1. The deduced amino acid sequence is homologous to channels of the mammalian two-pore domain halothane inhibited (THIK) subfamily, bearing 46% identity to THIK-1 (KCNK 13) and 48% to THIK-2 (KCNK12). We used in-situ hybridization to analyze the distribution of this class of channels in the CNS. AcK2p1 is specifically expressed in many central neurons of all major ganglia including the largest identified neurons MCC, R2 and LP1. The highest expression of AcK2p1 was detected in an asymmetrical and distinct cluster of up to 30 cells located at the dorsal-medial region of the right pleural ganglion. The neuron-specific distribution seen in the molluscan CNS is consistent with data from mammals that indicate THIK is only expressed in restricted neuronal populations, suggesting its involvement in both the maintenance of neuronal phenotype and in the specific functional role of these neurons in their respective networks.
Similar articles
-
Molecular characterization and expression of a two-pore domain potassium channel in the CNS of Aplysia californica.Brain Res. 2006 Jun 13;1094(1):47-56. doi: 10.1016/j.brainres.2006.03.122. Epub 2006 May 23. Brain Res. 2006. PMID: 16716269
-
Changes in expression of some two-pore domain potassium channel genes (KCNK) in selected brain regions of developing mice.Neuroscience. 2008 Feb 19;151(4):1154-72. doi: 10.1016/j.neuroscience.2007.12.011. Epub 2007 Dec 23. Neuroscience. 2008. PMID: 18222039
-
Single-channel properties and pH sensitivity of two-pore domain K+ channels of the TALK family.Biochem Biophys Res Commun. 2004 Mar 19;315(4):836-44. doi: 10.1016/j.bbrc.2004.01.137. Biochem Biophys Res Commun. 2004. PMID: 14985088
-
Potassium leak channels and the KCNK family of two-P-domain subunits.Nat Rev Neurosci. 2001 Mar;2(3):175-84. doi: 10.1038/35058574. Nat Rev Neurosci. 2001. PMID: 11256078 Review.
-
Organisation of potassium channels on the neuronal surface.J Chem Neuroanat. 2010 Sep;40(1):1-20. doi: 10.1016/j.jchemneu.2010.03.003. Epub 2010 Mar 23. J Chem Neuroanat. 2010. PMID: 20338235 Review.
Cited by
-
Neuronal transcriptome of Aplysia: neuronal compartments and circuitry.Cell. 2006 Dec 29;127(7):1453-67. doi: 10.1016/j.cell.2006.09.052. Cell. 2006. PMID: 17190607 Free PMC article.
-
Evolution of acid nociception: ion channels and receptors for detecting acid.Philos Trans R Soc Lond B Biol Sci. 2019 Nov 11;374(1785):20190291. doi: 10.1098/rstb.2019.0291. Epub 2019 Sep 23. Philos Trans R Soc Lond B Biol Sci. 2019. PMID: 31544616 Free PMC article. Review.
-
TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.J Neurosci. 2014 Jan 22;34(4):1494-509. doi: 10.1523/JNEUROSCI.4528-13.2014. J Neurosci. 2014. PMID: 24453337 Free PMC article.
-
Do different neurons age differently? Direct genome-wide analysis of aging in single identified cholinergic neurons.Front Aging Neurosci. 2010 May 19;2:6. doi: 10.3389/neuro.24.006.2010. eCollection 2010. Front Aging Neurosci. 2010. PMID: 20725513 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
