PLC-beta 3 signals upstream of PKC epsilon in acute and chronic inflammatory hyperalgesia
- PMID: 17350763
- DOI: 10.1016/j.pain.2007.01.027
PLC-beta 3 signals upstream of PKC epsilon in acute and chronic inflammatory hyperalgesia
Abstract
While protein kinase C epsilon has been shown to contribute to acute and chronic mechanical hyperalgesia, its upstream signaling pathway has received little attention. Since phospholipase C can signal to PKC epsilon and has been implicated in nociceptor sensitization, we tested if it is upstream of PKC epsilon in mechanisms underlying primary mechanical hyperalgesia. In the rat, the PKC epsilon-dependent mechanical hyperalgesia and hyperalgesic priming (i.e., a form of chronic latent enhanced hyperalgesia) induced by carrageenan were attenuated by a non-selective PLC inhibitor U-73122. A lipid mediator of PLC signaling, l-alpha-lysophosphatidylcholine produced dose-dependent mechanical hyperalgesia and hyperalgesic priming, which was attenuated by EAVSLKPT, a selective PKC epsilon inhibitor. However, U-73122 did not attenuate hyperalgesia induced by psi epsilon RACK, a selective PKC epsilon activator. Antisense to PLC-beta 3 isoform, which was found in small-diameter dorsal root ganglion neurons, also attenuated carrageenan-induced acute and chronic-latent hyperalgesia. These studies support the suggestion that PLC-beta 3 is an important upstream signaling molecule for PKC epsilon-mediated acute and chronic inflammatory pain.
Similar articles
-
Nociceptor subpopulations involved in hyperalgesic priming.Neuroscience. 2010 Feb 3;165(3):896-901. doi: 10.1016/j.neuroscience.2009.11.029. Epub 2009 Nov 18. Neuroscience. 2010. PMID: 19931357 Free PMC article.
-
Chronic hypersensitivity for inflammatory nociceptor sensitization mediated by the epsilon isozyme of protein kinase C.J Neurosci. 2000 Jun 15;20(12):4680-5. doi: 10.1523/JNEUROSCI.20-12-04680.2000. J Neurosci. 2000. PMID: 10844037 Free PMC article.
-
Hyperalgesic priming in the rat demonstrates marked sexual dimorphism.Pain. 2003 Sep;105(1-2):143-50. doi: 10.1016/s0304-3959(03)00175-1. Pain. 2003. PMID: 14499430
-
Critical role of nociceptor plasticity in chronic pain.Trends Neurosci. 2009 Dec;32(12):611-8. doi: 10.1016/j.tins.2009.07.007. Epub 2009 Sep 24. Trends Neurosci. 2009. PMID: 19781793 Free PMC article. Review.
-
PLC-epsilon: a shared effector protein in Ras-, Rho-, and G alpha beta gamma-mediated signaling.Mol Interv. 2003 Aug;3(5):273-80. doi: 10.1124/mi.3.5.273. Mol Interv. 2003. PMID: 14993441 Review.
Cited by
-
Targeting G protein-coupled receptor signalling by blocking G proteins.Nat Rev Drug Discov. 2018 Nov;17(11):789-803. doi: 10.1038/nrd.2018.135. Epub 2018 Sep 28. Nat Rev Drug Discov. 2018. PMID: 30262890 Free PMC article. Review.
-
Accounting for the delay in the transition from acute to chronic pain: axonal and nuclear mechanisms.J Neurosci. 2015 Jan 14;35(2):495-507. doi: 10.1523/JNEUROSCI.5147-13.2015. J Neurosci. 2015. PMID: 25589745 Free PMC article.
-
High-fat diet causes mechanical allodynia in the absence of injury or diabetic pathology.Sci Rep. 2022 Sep 1;12(1):14840. doi: 10.1038/s41598-022-18281-x. Sci Rep. 2022. PMID: 36050326 Free PMC article.
-
TRPA1 is functionally expressed primarily by IB4-binding, non-peptidergic mouse and rat sensory neurons.PLoS One. 2012;7(10):e47988. doi: 10.1371/journal.pone.0047988. Epub 2012 Oct 25. PLoS One. 2012. PMID: 23133534 Free PMC article.
-
Phospholipase Cβ3 Expressed in Mouse DRGs is Involved in Inflammatory and Postoperative Pain.J Pain Res. 2020 Dec 10;13:3371-3384. doi: 10.2147/JPR.S280565. eCollection 2020. J Pain Res. 2020. PMID: 33335421 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources