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. 2010 Mar 10;5(3):e9490.
doi: 10.1371/journal.pone.0009490.

FastTree 2--approximately maximum-likelihood trees for large alignments

Morgan N Price  1 , Paramvir S Dehal, Adam P Arkin
Affiliations

FastTree 2--approximately maximum-likelihood trees for large alignments

Morgan N Price et al. PLoS One. .

Abstract

Background: We recently described FastTree, a tool for inferring phylogenies for alignments with up to hundreds of thousands of sequences. Here, we describe improvements to FastTree that improve its accuracy without sacrificing scalability.

Methodology/principal findings: Where FastTree 1 used nearest-neighbor interchanges (NNIs) and the minimum-evolution criterion to improve the tree, FastTree 2 adds minimum-evolution subtree-pruning-regrafting (SPRs) and maximum-likelihood NNIs. FastTree 2 uses heuristics to restrict the search for better trees and estimates a rate of evolution for each site (the "CAT" approximation). Nevertheless, for both simulated and genuine alignments, FastTree 2 is slightly more accurate than a standard implementation of maximum-likelihood NNIs (PhyML 3 with default settings). Although FastTree 2 is not quite as accurate as methods that use maximum-likelihood SPRs, most of the splits that disagree are poorly supported, and for large alignments, FastTree 2 is 100-1,000 times faster. FastTree 2 inferred a topology and likelihood-based local support values for 237,882 distinct 16S ribosomal RNAs on a desktop computer in 22 hours and 5.8 gigabytes of memory.

Conclusions/significance: FastTree 2 allows the inference of maximum-likelihood phylogenies for huge alignments. FastTree 2 is freely available at http://www.microbesonline.org/fasttree.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Local support values for splits found by PhyML with SPR moves and/or FastTree.
We examined local support values for the splits inferred by PhyML 3.0 with formula image + SPRs on simulated alignments with 250 protein sequences. We classified PhyML's splits as correct and found by both PhyML and FastTree, correct but missed by FastTree, or incorrect. We show the distribution of support values for each class. The right-most bin includes the strongly supported splits (0.95 to 1.0), and the gray dashed line shows the uniform distribution. The support values are PhyML's minimum of the approximate likelihood ratio test and SH-like , local supports.
Figure 2
Figure 2. Likelihoods over time for genuine alignments.
Each line shows the time it takes a different tool to reach a given likelihood. For the COG alignments, all times and likelihoods are averages over the seven alignments. For FastTree, we show the time and the improvement in likelihood for the minimum-evolution topology and the final (approximately-ML) topology. For RAxML, we show the maximum parsimony starting topology, the first two rounds of SPR moves, and the final topology (note the interrupted formula image axis). For RAxML with FastTree's (minimum-evolution) starting tree, we show the starting topology and RAxML's first two rounds of SPR moves.
Figure 3
Figure 3. Traversing a tree with up-posteriors.
FastTree optimizes the tree near node N by analyzing the posterior distributions for subtrees A, B, and C, as well as the “up-posterior” D.
See this image and copyright information in PMC

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References

    1. Nawrocki EP, Kolbe DL, Eddy SR. Infernal 1.0: inference of RNA alignments. Bioinformatics. 2009;15:1335–7. - PMC - PubMed
    1. Price MN, Dehal PS, Arkin AP. FastTree: computing large minimum evolution trees with profiles instead of a distance matrix. Mol Biol Evol. 2009;26:1641–50. - PMC - PubMed
    1. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4:406–425. - PubMed
    1. Studier JA, Keppler KJ. A note on the neighbor-joining algorithm of Saitou and Nei. Mol Biol Evol. 1988;5:729–31. - PubMed
    1. Felsenstein J. Evolutionary trees from dna sequences: A maximum likelihood approach. J Mol Evol. 1981;17:368–376. - PubMed

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