A diet high in resistant starch modulates microbiota composition, SCFA concentrations, and gene expression in pig intestine
- PMID: 23325922
- DOI: 10.3945/jn.112.169672
A diet high in resistant starch modulates microbiota composition, SCFA concentrations, and gene expression in pig intestine
Abstract
Resistant starch (RS) is highly fermentable by microbiota in the colon, resulting in the production of SCFAs. RS is thought to mediate a large proportion of its health benefits, including increased satiety, through the actions of SCFAs. The aim of this study was to investigate the effects of a diet high in RS on luminal microbiota composition, luminal SCFA concentrations, and the expression of host genes involved in SCFA uptake, SCFA signaling, and satiety regulation in mucosal tissue obtained from small intestine, cecum, and colon. Twenty adult female pigs were either assigned to a digestible starch (DS) diet or a diet high in RS (34%) for a period of 2 wk. After the intervention, luminal content and mucosal scrapings were obtained for detailed molecular analysis. RS was completely degraded in the cecum. In both the cecum and colon, differences in microbiota composition were observed between DS- and RS-fed pigs. In the colon these included the stimulation of the healthy gut-associated butyrate-producing Faecalibacterium prausnitzii, whereas potentially pathogenic members of the Gammaproteobacteria, including Escherichia coli and Pseudomonas spp., were reduced in relative abundance. Cecal and colonic SCFA concentrations were significantly greater in RS-fed pigs, and cecal gene expression of monocarboxylate transporter 1 (SLC16A1) and glucagon (GCG) was induced by RS. In conclusion, our data show that RS modulates microbiota composition, SCFA concentrations, and host gene expression in pig intestine. Combined, our data provide an enhanced understanding of the interaction between diet, microbiota, and host.
Similar articles
-
Enzymatically modified starch up-regulates expression of incretins and sodium-coupled monocarboxylate transporter in jejunum of growing pigs.Animal. 2017 Jul;11(7):1180-1188. doi: 10.1017/S1751731116002615. Epub 2016 Dec 8. Animal. 2017. PMID: 27927266
-
Differential effects of dietary whey, casein and soya on colonic DNA damage and large bowel SCFA in rats fed diets low and high in resistant starch.Br J Nutr. 2007 Mar;97(3):535-43. doi: 10.1017/S0007114507336817. Br J Nutr. 2007. PMID: 17313716
-
Effects of potato fiber and potato-resistant starch on biomarkers of colonic health in rats fed diets containing red meat.J Food Sci. 2012 Oct;77(10):H216-23. doi: 10.1111/j.1750-3841.2012.02911.x. Epub 2012 Sep 5. J Food Sci. 2012. PMID: 22950602
-
Dietary resistant starch and chronic inflammatory bowel diseases.Int J Colorectal Dis. 1999 Nov;14(4-5):201-11. doi: 10.1007/s003840050212. Int J Colorectal Dis. 1999. PMID: 10647628 Review.
-
Role of intestinal bacteria in nutrient metabolism.JPEN J Parenter Enteral Nutr. 1997 Nov-Dec;21(6):357-65. doi: 10.1177/0148607197021006357. JPEN J Parenter Enteral Nutr. 1997. PMID: 9406136 Review.
Cited by
-
Application of resistant starch in swine and poultry diets with particular reference to gut health and function.Anim Nutr. 2018 Sep;4(3):305-310. doi: 10.1016/j.aninu.2018.04.001. Epub 2018 Apr 13. Anim Nutr. 2018. PMID: 30175259 Free PMC article. Review.
-
Cross-species comparison of genes related to nutrient sensing mechanisms expressed along the intestine.PLoS One. 2014 Sep 12;9(9):e107531. doi: 10.1371/journal.pone.0107531. eCollection 2014. PLoS One. 2014. PMID: 25216051 Free PMC article.
-
Gut Microbiota Secondary Metabolites: Key Roles in GI Tract Cancers and Infectious Diseases.Biomedicines. 2025 Jan 3;13(1):100. doi: 10.3390/biomedicines13010100. Biomedicines. 2025. PMID: 39857684 Free PMC article. Review.
-
Evidence for greater production of colonic short-chain fatty acids in overweight than lean humans.Int J Obes (Lond). 2014 Dec;38(12):1525-31. doi: 10.1038/ijo.2014.46. Epub 2014 Mar 19. Int J Obes (Lond). 2014. PMID: 24642959 Free PMC article.
-
Starch characterization of commercial extruded dry pet foods.Transl Anim Sci. 2020 Feb 14;4(2):txaa018. doi: 10.1093/tas/txaa018. eCollection 2020 Apr. Transl Anim Sci. 2020. PMID: 32705018 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous