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. 2013 Mar;21(2):126-31.
doi: 10.4062/biomolther.2013.014.

Identification of differentially expressed genes by gabapentin in cultured dorsal root ganglion in a rat neuropathic pain model

Ji Hye Heo  1 , Seung Ha Lee, Kyung Ha Chang, Eun Hye Han, Seung Gwan Lee, Dal Woong Choi, Suhng Wook Kim
Affiliations

Identification of differentially expressed genes by gabapentin in cultured dorsal root ganglion in a rat neuropathic pain model

Ji Hye Heo et al. Biomol Ther (Seoul). 2013 Mar.

Abstract

Neuropathic pain is a chronic pain disorder caused by nervous system lesions as a direct consequence of a lesion or by disease of the portions of the nervous system that normally signal pain. The spinal nerve ligation (SNL) model in rats that reflect some components of clinical pain have played a crucial role in the understanding of neuropathic pain. To investigate the direct effects of gabapentin on differential gene expression in cultured dorsal root ganglion (DRG) cells of SNL model rats, we performed a differential display reverse transcription-polymerase chain reaction analysis with random priming approach using annealing control primer. Genes encoding metallothionein 1a, transforming growth factor-β1 and palmitoyl-protein thioesterase-2 were up-regulated in gabapentin-treated DRG cells of SNL model rats. The functional roles of these differentially expressed genes were previously suggested as neuroprotective genes. Further study of these genes is expected to reveal potential targets of gabapentin.

Keywords: Dorsal root ganglion; Gabapentin; Neuropathic pain; Spinal nerve ligation.

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Figures

Fig. 1.
Fig. 1.. Morphology of cultured DRG cells for three days in the absence (A) and presence (B) of gabapentin. Magnification, ×100.
Fig. 2.
Fig. 2.. Detection of differentially expressed genes in cultured DRG cells treated with gabapentin. Total RNA was extracted from DRG cells treated with gabapentin and subjected to ACP-based DDRT-PCR. Twenty arbitrary ACP primers (ACP1 to ACP20) were used to isolate the differentially expressed genes. Differential expression patterns were observed when the arbitrary ACP primer sets (indicated on the top) were used. The differential expression patterns were evaluated based on the band intensities. The arrows on the left-hand side indicate differential expressed bands between untreated DRG cells (U) and gabapentin-treated DRG cells (G). Bands were excised from the gel for sequencing. M, 100-bp DNA ladder.
Fig. 3.
Fig. 3.. Confirmation by real-time RT-PCR of mRNA expression patterns of three selected genes. Real-time RT-PCR shows up-regulation of metallothionein 1a, TGF-1 and PPT-2 in cultured DRG cells treated with gabapentin. Data are shown as means +SD (bars) of samples conducted in triplicate determinations. To normalize the efficiency of real-time RT-PCR reaction, β-actin gene was used as an internal standard.
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References

    1. Abe M., Kurihara T., Han W., Shinomiya K., Tanabe T. Changes in expression of voltage-dependent ion channel subunits in dorsal root ganglia of rats with radicular injury and pain. Spine (Phila. Pa 1976) (2002);27:1517–1524. - PubMed
    1. Aldrich B. T., Frakes E. P., Kasuya J., Hammond D. L., Kitamoto T. Changes in expression of sensory organ-specific microRNAs in rat dorsal root ganglia in association with mechanical hypersensitivity induced by spinal nerve ligation. Neuroscience. (2009);164:711–723. - PMC - PubMed
    1. Baron R. Peripheral neuropathic pain: from mechanisms to symptoms. Clin. J. Pain. (2000);16:S12–20. - PubMed
    1. Bridges D., Thompson S. W., Rice A. S. Mechanisms of neuropathic pain. Br. J. Anaesth. (2001);87:12–26. - PubMed
    1. Brionne T. C., Tesseur I., Masliah E., Wyss-Coray T. Loss of TGF-beta 1 leads to increased neuronal cell death and microgliosis in mouse brain. Neuron. (2003);40:1133–1145. - PubMed

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