doi: 10.1073/pnas.83.6.1709.
Bovine parathyroid cells: cultures maintained for more than 140 population doublings
- PMID: 3006065
- PMCID: PMC323153
- DOI: 10.1073/pnas.83.6.1709
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Bovine parathyroid cells: cultures maintained for more than 140 population doublings
Proc Natl Acad Sci U S A.
1986 Mar.
Abstract
Primary cultures of bovine parathyroid cells were developed using Coon's modified Ham's F-12 medium containing low (0.3 mM) concentrations of calcium and supplements of bovine hypothalamic extract, bovine pituitary extract, epidermal growth factor, insulin, transferrin, selenous acid, hydrocortisone, triiodothyronine, retinoic acid, and galactose. These cells were cultured serially on serum-coated dishes for 140 population doublings before signs of senescence were detected. The cells were epithelioid and maintained a high degree of differentiation as evidenced by calcium regulation of both growth and secretion and by prostaglandin E1 stimulation of cAMP formation and hormone release.
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