Segmental muscle fiber lesions after repetitive eccentric contractions
- PMID: 9634608
- DOI: 10.1007/s004410051108
Segmental muscle fiber lesions after repetitive eccentric contractions
Abstract
Immunohistochemical and electron-microscopic techniques were used to analyze the extensor digitorum longus muscles of New Zealand White rabbits 1 h, 1 day, 3, 7, and 28 days after repetitive eccentric contractions. Loss of the cytoskeletal protein desmin was the earliest manifestation of injury. Apart from 1 h post-exercise, all desmin-negative fibers stained positively with antibody to plasma fibronectin, indicating loss of cellular integrity accompanying cytoskeletal disruption. Fiber sizes were significantly increased from 1-7 days after exercise. The large (hyaline) fibers found in histological sections after repetitive eccentric contractions resulted from segmental hypercontraction of the fiber. This phenomenon occurred proximally and distally to plasma membrane lesions of the muscle fiber and necrosis and manifested itself as very short sarcomere lengths. Thus, in serial sections, staining characteristics, sizes and shapes of one and the same fiber often varied dramatically. We conclude that the following sequence of events occurs: cytoskeletal disruptions, loss of myofibrillar registry, i.e., Z-disk streaming and A-band disorganization, and loss of cell integrity as manifested by intracellular plasma fibronectin stain, hypercontracted regions, and invasion of cells. When a fiber is disrupted, the remaining intact fibers apparently take up the tension put on the muscle and later fewer fibers are subjected to eccentric contractions.
Similar articles
-
Muscle cytoskeletal disruption occurs within the first 15 min of cyclic eccentric contraction.J Appl Physiol (1985). 1996 Jan;80(1):278-84. doi: 10.1152/jappl.1996.80.1.278. J Appl Physiol (1985). 1996. PMID: 8847315
-
Biodiversity of the localization of the epitopes to connectin antibodies in the sarcomeres of lamprey, electric ray, and horse mackerel skeletal muscles.Tissue Cell. 1994 Oct;26(5):677-85. doi: 10.1016/0040-8166(94)90052-3. Tissue Cell. 1994. PMID: 9437246
-
Contractile and cellular remodeling in rabbit skeletal muscle after cyclic eccentric contractions.J Appl Physiol (1985). 1994 Oct;77(4):1926-34. doi: 10.1152/jappl.1994.77.4.1926. J Appl Physiol (1985). 1994. PMID: 7836220
-
Viscoelasticity and function of connectin/titin filaments in skinned muscle fibers.Adv Biophys. 1996;33:159-71. doi: 10.1016/s0065-227x(96)90031-3. Adv Biophys. 1996. PMID: 8922111 Review. No abstract available.
-
Cytoskeletal disruption after eccentric contraction-induced muscle injury.Clin Orthop Relat Res. 2002 Oct;(403 Suppl):S90-9. doi: 10.1097/00003086-200210001-00011. Clin Orthop Relat Res. 2002. PMID: 12394457 Review.
Cited by
-
An expansion of Simons' integrated hypothesis of trigger point formation.Curr Pain Headache Rep. 2004 Dec;8(6):468-75. doi: 10.1007/s11916-004-0069-x. Curr Pain Headache Rep. 2004. PMID: 15509461 Review.
-
Treatment with selectin blocking antibodies after lengthening contractions of mouse muscle blunts neutrophil accumulation but does not reduce damage.Physiol Rep. 2016 Jan;4(1):e12667. doi: 10.14814/phy2.12667. Physiol Rep. 2016. PMID: 26733249 Free PMC article.
-
Etiology of myofascial trigger points.Curr Pain Headache Rep. 2012 Oct;16(5):439-44. doi: 10.1007/s11916-012-0289-4. Curr Pain Headache Rep. 2012. PMID: 22836591 Free PMC article. Review.
-
Repeated bout effect on the cytoskeletal proteins titin, desmin, and dystrophin in rat skeletal muscle.J Muscle Res Cell Motil. 2007;28(1):39-47. doi: 10.1007/s10974-007-9102-0. Epub 2007 Apr 14. J Muscle Res Cell Motil. 2007. PMID: 17436058
-
Contractile properties of EDL and soleus muscles of myostatin-deficient mice.J Appl Physiol (1985). 2006 Sep;101(3):898-905. doi: 10.1152/japplphysiol.00126.2006. Epub 2006 May 18. J Appl Physiol (1985). 2006. PMID: 16709649 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
