doi: 10.1111/j.1574-6968.2000.tb09243.x.
S-layer gene as a molecular marker for identification of Lactobacillus helveticus
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- PMID: 10930751
- DOI: 10.1111/j.1574-6968.2000.tb09243.x
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S-layer gene as a molecular marker for identification of Lactobacillus helveticus
FEMS Microbiol Lett.
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Abstract
The paper describes two methods that allow rapid and accurate identification of Lactobacillus helveticus strains based on the nucleotide sequence of the gene coding for the surface layer (S-layer) protein. The first method is based on a polymerase chain reaction amplification using primers targeting a specific fragment of the S-layer gene. The second method involved a portion of the S-layer gene of L. helveticus as a probe in a hybridisation test. The specificity of these protocols was checked for DNA samples isolated from various Lactobacillus strains.
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