Butyrate modulates oxidative stress in the colonic mucosa of healthy humans
- PMID: 19108937
- DOI: 10.1016/j.clnu.2008.11.002
Butyrate modulates oxidative stress in the colonic mucosa of healthy humans
Abstract
Background & aims: Butyrate, a short-chain fatty acid produced by colonic microbial fermentation of undigested carbohydrates, has been implicated in the maintenance of colonic health. This study evaluates whether butyrate plays a role in oxidative stress in the healthy colonic mucosa.
Methods: A randomized, double blind, cross-over study with 16 healthy volunteers was performed. Treatments consisted of daily rectal administration of a 60 ml enema containing 100 mM sodium butyrate or saline for 2 weeks. After each treatment, a blood sample was taken and mucosal biopsies were obtained from the sigmoid colon. In biopsies, the trolox equivalent antioxidant capacity, activity of glutathione-S-transferase, concentration of uric acid, glutathione (GSH), glutathione disulfide and malondialdehyde, and expression of genes involved in GSH and uric acid metabolism was determined. Secondary outcome parameters were CRP, calprotectin and intestinal fatty acid binding protein in plasma and histological inflammatory scores.
Results: Butyrate treatment resulted in significantly higher GSH (p<0.05) and lower uric acid (p<0.01) concentrations compared to placebo. Changes in GSH and uric acid were accompanied by increased and decreased expression, respectively, of their rate limiting enzymes determined by RT-PCR. No significant differences were found in other parameters.
Conclusions: This study demonstrated that butyrate is able to beneficially affect oxidative stress in the healthy human colon.
Trial registration: ClinicalTrials.gov NCT00693355.
Similar articles
-
Review article: the role of butyrate on colonic function.Aliment Pharmacol Ther. 2008 Jan 15;27(2):104-19. doi: 10.1111/j.1365-2036.2007.03562.x. Epub 2007 Oct 25. Aliment Pharmacol Ther. 2008. PMID: 17973645 Review.
-
The effects of butyrate enemas on visceral perception in healthy volunteers.Neurogastroenterol Motil. 2009 Sep;21(9):952-e76. doi: 10.1111/j.1365-2982.2009.01324.x. Epub 2009 May 19. Neurogastroenterol Motil. 2009. PMID: 19460106 Clinical Trial.
-
Effect of butyrate enemas on inflammation and antioxidant status in the colonic mucosa of patients with ulcerative colitis in remission.Clin Nutr. 2010 Dec;29(6):738-44. doi: 10.1016/j.clnu.2010.04.002. Epub 2010 May 15. Clin Nutr. 2010. PMID: 20471725 Review.
-
Comparisons of the anti-proliferative effects of butyrate and aspirin on human colonic mucosa in vitro.Eur J Cancer Prev. 2000 Jun;9(3):205-11. Eur J Cancer Prev. 2000. PMID: 10954260
-
Butyrate enemas do not affect human colonic MUC2 and TFF3 expression.Eur J Gastroenterol Hepatol. 2010 Sep;22(9):1134-40. doi: 10.1097/MEG.0b013e32833a6ca0. Eur J Gastroenterol Hepatol. 2010. PMID: 20461009 Clinical Trial.
Cited by
-
Low iron availability in continuous in vitro colonic fermentations induces strong dysbiosis of the child gut microbial consortium and a decrease in main metabolites.FEMS Microbiol Ecol. 2013 Jan;83(1):161-75. doi: 10.1111/j.1574-6941.2012.01461.x. Epub 2012 Aug 28. FEMS Microbiol Ecol. 2013. PMID: 22845175 Free PMC article.
-
Next-Generation Probiotics: Microflora Intervention to Human Diseases.Biomed Res Int. 2022 Nov 16;2022:5633403. doi: 10.1155/2022/5633403. eCollection 2022. Biomed Res Int. 2022. PMID: 36440358 Free PMC article. Review.
-
Effect of Size and Loading of Retinoic Acid in Polyvinyl Butyrate Nanoparticles on Amelioration of Colitis.Polymers (Basel). 2021 May 2;13(9):1472. doi: 10.3390/polym13091472. Polymers (Basel). 2021. PMID: 34063206 Free PMC article.
-
Microbial degradation of whole-grain complex carbohydrates and impact on short-chain fatty acids and health.Adv Nutr. 2015 Mar 13;6(2):206-13. doi: 10.3945/an.114.007450. Print 2015 Mar. Adv Nutr. 2015. PMID: 25770259 Free PMC article. Review.
-
Reevaluating the hype: four bacterial metabolites under scrutiny.Eur J Microbiol Immunol (Bp). 2015 Mar;5(1):1-13. doi: 10.1556/EUJMI-D-14-00030. Epub 2015 Mar 26. Eur J Microbiol Immunol (Bp). 2015. PMID: 25883790 Free PMC article. Review.
Publication types
MeSH terms
Substances
Associated data
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous