| Official Gene Symbol | Other Aliases |
|---|---|
| CAPG | AFCP, MCP |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 348 | 38499 |
| Protein Name |
|---|
| Macrophage-capping protein |
| Sources | |
|---|---|
|
UniProt PhosphoSitePlus ® GeneCards |
Human Protein Atlas |
| 10 | 20 | 30 | 40 | 50 |
| MYTAIPQSGS | PFPGSVQDPG | LHVWRVEKLK | PVPVAQENQG | VFFSGDSYLV |
| 60 | 70 | 80 | 90 | 100 |
| LHNGPEEVSH | LHLWIGQQSS | RDEQGACAVL | AVHLNTLLGE | RPVQHREVQG |
| 110 | 120 | 130 | 140 | 150 |
| NESDLFMSYF | PRGLKYQEGG | VESAFHKTST | GAPAAIKKLY | QVKGKKNIRA |
| 160 | 170 | 180 | 190 | 200 |
| TERALNWDSF | NTGDCFILDL | GQNIFAWCGG | KSNILERNKA | RDLALAIRDS |
| 210 | 220 | 230 | 240 | 250 |
| ERQGKAQVEI | VTDGEEPAEM | IQVLGPKPAL | KEGNPEEDLT | ADKANAQAAA |
| 260 | 270 | 280 | 290 | 300 |
| LYKVSDATGQ | MNLTKVADSS | PFALELLISD | DCFVLDNGLC | GKIYIWKGRK |
| 310 | 320 | 330 | 340 | 348 |
| ANEKERQAAL | QVAEGFISRM | QYAPNTQVEI | LPQGHESPIF | KQFFKDWK |
Data source: UniProt
Uniprot Database Entry PhosphoSitePlus ®
loading
Data source: Panorama
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 11.3 | 5.4 | 4.8 | 15.1 | 7.5 | 5 | 18.9 | 9.2 | 6.9 | 15 | 25 | 15 |
| y8 (1+) | 23.2 | 7.3 | 4.5 | 29.4 | 8.9 | 6.7 | 37.5 | 11.5 | 8.1 | 15 | 25 | 15 |
| y7 (1+) | 14.5 | 7.7 | 7.1 | 17.9 | 8.5 | 8.9 | 23 | 11.5 | 11.4 | 15 | 25 | 15 |
| sum | 9.8 | 5.3 | 5.2 | 15.5 | 7.5 | 5.6 | 18.3 | 9.2 | 7.6 | 15 | 25 | 15 |
Data source: Panorama
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 6.8 | 5.5 | 5.2 | 6.6 | 4.9 | 10.8 | 9.5 | 7.4 | 12 | 15 | 25 | 15 |
| y5 (1+) | 5.2 | 13.4 | 11 | 5.7 | 14.8 | 21 | 7.7 | 20 | 23.7 | 15 | 25 | 15 |
| y9 (2+) | 10.3 | 6.5 | 8.7 | 14.1 | 5.4 | 9.3 | 17.5 | 8.5 | 12.7 | 15 | 25 | 15 |
| sum | 3.6 | 8.3 | 5.5 | 4 | 9.3 | 9 | 5.4 | 12.5 | 10.5 | 15 | 25 | 15 |
")
.appendTo(all_in_one_graph)
.click(function (e) {
e.preventDefault();
all_in_one_plot.pan(offset);
});
}
addArrow("left", 55, 40, {left: -100});
addArrow("right", 25, 40, {left: 100});
addArrow("up", 40, 25, {top: -100});
addArrow("down", 40, 55, {top: 100});
// Disable the mouse wheel functionality for panning and zooming
$('#all_in_one_graph canvas.flot-overlay').unmousewheel();
$(all_in_one_graph).bind("plotclick", function (event, pos, item) {
// Splice Junctions
// http://www.uniprot.org/uniprot/P04626#section_features
if ((item != null) && (item.series.data[0][3] != null) && (item.series.data[0][3] == 'splice_junction')) {
var url = 'http://www.uniprot.org/uniprot/' + uniprot_id + '#section_features';
window.open(url, '_blank');
}
// Isoforms
if ((item != null) && (item.series.data[0][4] != null) && (item.series.data[0][4] == 'isoform')) {
var url = 'http://www.uniprot.org/uniprot/' + item.series.data[item.dataIndex][3];
window.open(url, '_blank');
}
// SNPs
if ((item != null) && (item.series.data[0][3] != null) && (item.series.data[0][3] == 'snp')) {
var url = 'http://www.uniprot.org/blast/?about=' + uniprot_id + '[' + item.datapoint[0] + ']';
window.open(url, '_blank');
}
// MRM Assays
if ((item != null) && (item.series.data[0][4] != null) && (item.series.data[0][4] == 'mrm_assay')) {
var this_peptide_sequence = item.series.data[item.dataIndex][3];
var this_protein_id = item.series.data[item.dataIndex][5];
var div_id = '.' + this_protein_id;
// Change our States (URL, title in title bar)
History.replaceState({state: 1}, "CAPG, CPTAC-" + this_protein_id + " - CPTAC Assay Portal", "CPTAC-" + this_protein_id);
// Scroll to assays area
scrollToAnchor('assay_details_anchor');
$('.assay-details-wrapper').hide();
$('#preloader_loading_assay_details').fadeIn(500);
$('#preloader_loading_assay_details').hide();
$(div_id).fadeIn(300);
// Load Disqus commenting
//loadDisqus($('#comments_container_' + this_protein_id), this_peptide_sequence, 'http://assays.cancer.gov/CPTAC-327/#!' + this_peptide_sequence);
}
});
///////////////////////////////////////////////////////////
// [BEGIN] Highlighted Peptide Sequence Links ///////////////
// Use sequence values from the 'genes_distinct' array
var mouseY;
var mouseX;
$(document).mousemove(function (e) {
mouseX = e.pageX;
mouseY = e.pageY;
});
var starts = JSON.parse('[{"peptide_sequence":"EGNPEEDLTADK","manage":328},{"peptide_sequence":"YQEGGVESAFHK","manage":327}]');
var last_clicked = false;
$.each(starts, function (index, starts_data) {
// On click event for peptide sequence links in the gene sequence
var this_peptide_sequence = starts_data.peptide_sequence;
var this_protein_id = starts_data.manage;
var div_id = '.' + this_protein_id;
var clickable_class = 'seq-' + this_peptide_sequence;
$('.' + clickable_class).on('click', function () {
$("rect, line, .close").tooltip();
if (last_clicked != this_peptide_sequence) {
$('.detail-modal').attr('style', 'display: none');
last_clicked = false;
}
var div_height = $('.all-details-' + this_peptide_sequence).height();
var offset = (div_height > 25) ? 450 : 390;
$('.all-details-' + this_peptide_sequence).css({'top': mouseY - offset}).fadeIn('fast');
$('.detail-modal .close').on('click', function () {
$('.detail-modal').fadeOut('fast');
});
});
var detail_link_class = this_peptide_sequence + '-' + this_protein_id;
$('.protein-sequence').on('click', '.' + detail_link_class, function () {
// close the pop up
$('.detail-modal').fadeOut('fast');
// Change our States (URL, title in title bar)
History.replaceState({state: 1}, "CAPG, CPTAC-" + this_protein_id + " - CPTAC Assay Portal", "CPTAC-" + this_protein_id);
// Scroll to assays area
scrollToAnchor('assay_details_anchor');
$('.assay-details-wrapper').hide();
$('#preloader_loading_assay_details').fadeIn(500);
$('#preloader_loading_assay_details').hide();
$(div_id).fadeIn(300);
// Load Disqus commenting
//loadDisqus($('#comments_container_' + this_protein_id), this_peptide_sequence, 'http://assays.cancer.gov/CPTAC-327/#!' + this_peptide_sequence);
});
});
// $("rect, line, .peptide_highlight").tooltip({
// 'container': 'body',
// 'placement': 'top'
// });
// [END] Highlighted Peptide Sequence Links ///////////////
// Tooltips on hover of data points
var previousPoint = null;
$(all_in_one_graph).bind("plothover", function (event, pos, item) {
if (item) {
document.body.style.cursor = 'pointer';
if (previousPoint != item.dataIndex) {
previousPoint = item.dataIndex;
$("#tooltip").remove();
showTooltip(item.pageX, item.pageY, item.series.data[item.dataIndex][2]);
}
} else {
document.body.style.cursor = 'default';
$("#tooltip").remove();
previousPoint = null;
}
});
// Pop-out entire sequence
$('div.sequence').on('mouseenter', '.protein-sequence', function (event) {
$(this).attr('style', 'overflow: visible;');
$('table.sequence').addClass('sequence_table_shadow');
});
$('div.sequence').on('mouseleave', '.protein-sequence', function (event) {
$(this).attr('style', 'overflow: hidden;');
$('table.sequence').removeClass('sequence_table_shadow');
$('.detail-modal').attr('style', 'display: none');
});
// Tooltips
$('.span4 img, .chart-legend, .chart-legend-text').tooltip();
// Show the Assay details for the page id we're on
var details_div_id = $('#outer-wrapper .327');
$(details_div_id).show();
//loadDisqus($('#comments_container_327'), 'YQEGGVESAFHK', 'http://assays.cancer.gov/CPTAC-327/#!YQEGGVESAFHK');
// History.js
(function (window, undefined) {
// Bind to StateChange Event
History.Adapter.bind(window, 'statechange', function () { // Note: We are using statechange instead of popstate
var State = History.getState(); // Note: We are using History.getState() instead of event.state
});
})(window);
$.ajax({
type: "GET"
, dataType: "html"
, url: "/assays/get_protein_map_svg"
, data: ({uniprot_ac_id: uniprot_id})
, success: function (svg_return) {
d3.select("#proteincartoon").html(svg_return);
}
});
$('#proteincartoon').click(function () {
var win = window.open('http://www.phosphosite.org/uniprotAccAction.do?id=' + uniprot_id, '_blank');
win.focus();
});
});
// FUNCTIONS ///////////////
function scrollToAnchor(aid) {
var aTag = $("a[name='" + aid + "']");
$('html,body').animate({scrollTop: aTag.offset().top}, 'slow');
}
function showTooltip(x, y, contents) {
$("Table data unavailable
'; // Display the preloaders $.each(genes, function (index, gene) { $('div.span12.' + gene.peptide_sequence + '_' + gene.laboratory_abbreviation + ' .loc_lloq_preloader_loading').show(); }); // Send the request via AJAX $.ajax({ url: "/assays/get_plots_table_data" , dataType: "json" , type: "post" , data: {genes: JSON.stringify(genes)} , success: function (data) { if (data) { $.each(data, function (index, single_gene_data) { /* * Build out the LOC/LOQ data table */ if (single_gene_data.lod_loq_comparison_data[0].length) { var crude = (single_gene_data.peptide_standard_purity_types_id == 3) ? true : false; var peptide_sequence = single_gene_data.lod_loq_comparison_data[0][0].peptide; var lod_loq_units = single_gene_data.lod_loq_comparison_data[0][0].lod_loq_units; var crude_style = (crude) ? 'class="crude-header"' : ''; var lod_loq_units_note = (crude) ? 'Response Curves Data Unavailable
'); }); } }); } } }); }
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