| Official Gene Symbol | Other Aliases |
|---|---|
| Gfap | N/A |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 430 | 49900 |
| Protein Name |
|---|
| Glial fibrillary acidic protein |
| Sources | |
|---|---|
|
UniProt PhosphoSitePlus ® GeneCards |
Human Protein Atlas |
| 10 | 20 | 30 | 40 | 50 |
| MERRRITSAR | RSYASETVVR | GLGPSRQLGT | MPRFSLSRMT | PPLPARVDFS |
| 60 | 70 | 80 | 90 | 100 |
| LAGALNAGFK | ETRASERAEM | MELNDRFASY | IEKVRFLEQQ | NKALAAELNQ |
| 110 | 120 | 130 | 140 | 150 |
| LRAKEPTKLA | DVYQAELREL | RLRLDQLTAN | SARLEVERDN | FAQDLGTLRQ |
| 160 | 170 | 180 | 190 | 200 |
| KLQDETNLRL | EAENNLAAYR | QEADEATLAR | VDLERKVESL | EEEIQFLRKI |
| 210 | 220 | 230 | 240 | 250 |
| YEEEVRELRE | QLAQQQVHVE | MDVAKPDLTA | ALREIRTQYE | AVATSNMQET |
| 260 | 270 | 280 | 290 | 300 |
| EEWYRSKFAD | LTDAASRNAE | LLRQAKHEAN | DYRRQLQALT | CDLESLRGTN |
| 310 | 320 | 330 | 340 | 350 |
| ESLERQMREQ | EERHARESAS | YQEALARLEE | EGQSLKEEMA | RHLQEYQDLL |
| 360 | 370 | 380 | 390 | 400 |
| NVKLALDIEI | ATYRKLLEGE | ENRITIPVQT | FSNLQIRETS | LDTKSVSEGH |
| 410 | 420 | 430 | ||
| LKRNIVVKTV | EMRDGEVIKD | SKQEHKDVVM |
Data source: UniProt
Uniprot Database Entry PhosphoSitePlus ®
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Data source: Panorama
View Details (opens in a new window)
Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.
Data source: Panorama
Data source: Panorama
Data source: Panorama
Retrieving Data
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 9.4 | 5.6 | 5.4 | 11.1 | 10.4 | 6.8 | 14.5 | 11.8 | 8.7 | 15 | 15 | 15 |
| b3 (1+) | 9.1 | 6.1 | 4.7 | 13.5 | 8.4 | 5.2 | 16.3 | 10.4 | 7 | 15 | 15 | 15 |
| y6 (1+) | 13 | 6.2 | 5.3 | 20 | 8.4 | 4.9 | 23.9 | 10.4 | 7.2 | 15 | 15 | 15 |
| y4 (1+) | 20.3 | 6.7 | 4.6 | 30.9 | 8.3 | 5.3 | 37 | 10.7 | 7 | 15 | 15 | 15 |
| y5 (1+) | 9.5 | 5.2 | 4 | 12 | 10.6 | 6 | 15.3 | 11.8 | 7.2 | 15 | 15 | 15 |
| sum | 6.3 | 2.8 | 2.3 | 13.6 | 7.8 | 2.9 | 15 | 8.3 | 3.7 | 15 | 15 | 15 |
")
.appendTo(all_in_one_graph)
.click(function (e) {
e.preventDefault();
all_in_one_plot.pan(offset);
});
}
addArrow("left", 55, 40, {left: -100});
addArrow("right", 25, 40, {left: 100});
addArrow("up", 40, 25, {top: -100});
addArrow("down", 40, 55, {top: 100});
// Disable the mouse wheel functionality for panning and zooming
$('#all_in_one_graph canvas.flot-overlay').unmousewheel();
$(all_in_one_graph).bind("plotclick", function (event, pos, item) {
// Splice Junctions
// http://www.uniprot.org/uniprot/P04626#section_features
if ((item != null) && (item.series.data[0][3] != null) && (item.series.data[0][3] == 'splice_junction')) {
var url = 'http://www.uniprot.org/uniprot/' + uniprot_id + '#section_features';
window.open(url, '_blank');
}
// Isoforms
if ((item != null) && (item.series.data[0][4] != null) && (item.series.data[0][4] == 'isoform')) {
var url = 'http://www.uniprot.org/uniprot/' + item.series.data[item.dataIndex][3];
window.open(url, '_blank');
}
// SNPs
if ((item != null) && (item.series.data[0][3] != null) && (item.series.data[0][3] == 'snp')) {
var url = 'http://www.uniprot.org/blast/?about=' + uniprot_id + '[' + item.datapoint[0] + ']';
window.open(url, '_blank');
}
// MRM Assays
if ((item != null) && (item.series.data[0][4] != null) && (item.series.data[0][4] == 'mrm_assay')) {
var this_peptide_sequence = item.series.data[item.dataIndex][3];
var this_protein_id = item.series.data[item.dataIndex][5];
var div_id = '.' + this_protein_id;
// Change our States (URL, title in title bar)
History.replaceState({state: 1}, "Gfap, CPTAC-" + this_protein_id + " - CPTAC Assay Portal", "CPTAC-" + this_protein_id);
// Scroll to assays area
scrollToAnchor('assay_details_anchor');
$('.assay-details-wrapper').hide();
$('#preloader_loading_assay_details').fadeIn(500);
$('#preloader_loading_assay_details').hide();
$(div_id).fadeIn(300);
// Load Disqus commenting
//loadDisqus($('#comments_container_' + this_protein_id), this_peptide_sequence, 'http://assays.cancer.gov/non-CPTAC-3312/#!' + this_peptide_sequence);
}
});
///////////////////////////////////////////////////////////
// [BEGIN] Highlighted Peptide Sequence Links ///////////////
// Use sequence values from the 'genes_distinct' array
var mouseY;
var mouseX;
$(document).mousemove(function (e) {
mouseX = e.pageX;
mouseY = e.pageY;
});
var starts = JSON.parse('[{"peptide_sequence":"LADVYQAELR","manage":3312}]');
var last_clicked = false;
$.each(starts, function (index, starts_data) {
// On click event for peptide sequence links in the gene sequence
var this_peptide_sequence = starts_data.peptide_sequence;
var this_protein_id = starts_data.manage;
var div_id = '.' + this_protein_id;
var clickable_class = 'seq-' + this_peptide_sequence;
$('.' + clickable_class).on('click', function () {
$("rect, line, .close").tooltip();
if (last_clicked != this_peptide_sequence) {
$('.detail-modal').attr('style', 'display: none');
last_clicked = false;
}
var div_height = $('.all-details-' + this_peptide_sequence).height();
var offset = (div_height > 25) ? 450 : 390;
$('.all-details-' + this_peptide_sequence).css({'top': mouseY - offset}).fadeIn('fast');
$('.detail-modal .close').on('click', function () {
$('.detail-modal').fadeOut('fast');
});
});
var detail_link_class = this_peptide_sequence + '-' + this_protein_id;
$('.protein-sequence').on('click', '.' + detail_link_class, function () {
// close the pop up
$('.detail-modal').fadeOut('fast');
// Change our States (URL, title in title bar)
History.replaceState({state: 1}, "Gfap, CPTAC-" + this_protein_id + " - CPTAC Assay Portal", "CPTAC-" + this_protein_id);
// Scroll to assays area
scrollToAnchor('assay_details_anchor');
$('.assay-details-wrapper').hide();
$('#preloader_loading_assay_details').fadeIn(500);
$('#preloader_loading_assay_details').hide();
$(div_id).fadeIn(300);
// Load Disqus commenting
//loadDisqus($('#comments_container_' + this_protein_id), this_peptide_sequence, 'http://assays.cancer.gov/non-CPTAC-3312/#!' + this_peptide_sequence);
});
});
// $("rect, line, .peptide_highlight").tooltip({
// 'container': 'body',
// 'placement': 'top'
// });
// [END] Highlighted Peptide Sequence Links ///////////////
// Tooltips on hover of data points
var previousPoint = null;
$(all_in_one_graph).bind("plothover", function (event, pos, item) {
if (item) {
document.body.style.cursor = 'pointer';
if (previousPoint != item.dataIndex) {
previousPoint = item.dataIndex;
$("#tooltip").remove();
showTooltip(item.pageX, item.pageY, item.series.data[item.dataIndex][2]);
}
} else {
document.body.style.cursor = 'default';
$("#tooltip").remove();
previousPoint = null;
}
});
// Pop-out entire sequence
$('div.sequence').on('mouseenter', '.protein-sequence', function (event) {
$(this).attr('style', 'overflow: visible;');
$('table.sequence').addClass('sequence_table_shadow');
});
$('div.sequence').on('mouseleave', '.protein-sequence', function (event) {
$(this).attr('style', 'overflow: hidden;');
$('table.sequence').removeClass('sequence_table_shadow');
$('.detail-modal').attr('style', 'display: none');
});
// Tooltips
$('.span4 img, .chart-legend, .chart-legend-text').tooltip();
// Show the Assay details for the page id we're on
var details_div_id = $('#outer-wrapper .3312');
$(details_div_id).show();
//loadDisqus($('#comments_container_3312'), 'LADVYQAELR', 'http://assays.cancer.gov/non-CPTAC-3312/#!LADVYQAELR');
// History.js
(function (window, undefined) {
// Bind to StateChange Event
History.Adapter.bind(window, 'statechange', function () { // Note: We are using statechange instead of popstate
var State = History.getState(); // Note: We are using History.getState() instead of event.state
});
})(window);
$.ajax({
type: "GET"
, dataType: "html"
, url: "/assays/get_protein_map_svg"
, data: ({uniprot_ac_id: uniprot_id})
, success: function (svg_return) {
d3.select("#proteincartoon").html(svg_return);
}
});
$('#proteincartoon').click(function () {
var win = window.open('http://www.phosphosite.org/uniprotAccAction.do?id=' + uniprot_id, '_blank');
win.focus();
});
});
// FUNCTIONS ///////////////
function scrollToAnchor(aid) {
var aTag = $("a[name='" + aid + "']");
$('html,body').animate({scrollTop: aTag.offset().top}, 'slow');
}
function showTooltip(x, y, contents) {
$("Table data unavailable
'; // Display the preloaders $.each(genes, function (index, gene) { $('div.span12.' + gene.peptide_sequence + '_' + gene.laboratory_abbreviation + ' .loc_lloq_preloader_loading').show(); }); // Send the request via AJAX $.ajax({ url: "/assays/get_plots_table_data" , dataType: "json" , type: "post" , data: {genes: JSON.stringify(genes)} , success: function (data) { if (data) { $.each(data, function (index, single_gene_data) { /* * Build out the LOC/LOQ data table */ if (single_gene_data.lod_loq_comparison_data[0].length) { var crude = (single_gene_data.peptide_standard_purity_types_id == 3) ? true : false; var peptide_sequence = single_gene_data.lod_loq_comparison_data[0][0].peptide; var lod_loq_units = single_gene_data.lod_loq_comparison_data[0][0].lod_loq_units; var crude_style = (crude) ? 'class="crude-header"' : ''; var lod_loq_units_note = (crude) ? 'Response Curves Data Unavailable
'); }); } }); } } }); }
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