Echovirus 30 Induced Neuronal Cell Death through TRIO-RhoA Signaling Activation
Figure 1
Echo30 infection induced neuronal cell death.
Human neuroblastoma SK-N-SH cells at 80% confluence were infected with Echo30 with 2% FBS contained MEM at 48 hrs. (A) The effect of Echo30 infection on SK-N-SH cells. Echo30 infected SK-N-SH cells significantly increase cytopathic effect. (B) Measurement of cell viability by WST-1 assay. The viability of SK-N-SH cells was decreased by Echo30 infection (mean±S.E.M, *p<0.05). (C) Measurement of sub-G1 phase using flow cytometry analysis. Echo30 infection increase sub-G1 phase at cell cycle distribution. (D) Expression of apoptotic proteins using immunoblot analysis. The expression level of pro-apoptotic protein was increased by Echo30 infection. All data are representative of three independent experiments.