Remodeling of Tight Junctions and Enhancement of Barrier Integrity of the CACO-2 Intestinal Epithelial Cell Layer by Micronutrients
Fig 2
The effect of six micronutrients on CACO-2 transepithelial flux of 14C-D-Mannitol.
In all cases, after electrical measurements, the same CACO-2 cell layers represented in Fig 1 were used to perform radiotracer flux studies with 0.1mM, 0.20 μCi/ml 14C-D-mannitol, as described in Materials and Methods. Data represents the percent of control flux rate normalized for each experiment, and is expressed as the mean ± standard error for the total number of cell layers per condition, as detailed in Fig 1. * indicates P < 0.05, ** indicates P < 0.01 and *** indicates P < 0.001 (one-way ANOVA followed by Dunnett’s post hoc testing versus control).