Remodeling of Tight Junctions and Enhancement of Barrier Integrity of the CACO-2 Intestinal Epithelial Cell Layer by Micronutrients
Fig 4
Effect of zinc on CACO-2 transepithelial electrical resistance as a function of the differentiation state of the CACO-2 cell layer.
3-day, 7-day, and 21-day CACO-2 cell layers were treated on the apical and basal-lateral sides with 100μM zinc for 48 hrs before electrical measurements. Data represents the percentage of normalized control resistance (2 experiments, 4 cell layers per condition per experiment) as a function of zinc treatment. Data shown is expressed as the mean ± standard error of 8 cell layers per condition. ** indicates P < 0.01 (21-day vs. 3-day); *** indicates P < 0.001 (one-way ANOVA followed by Dunnett’s post hoc testing versus day 3).