Remodeling of Tight Junctions and Enhancement of Barrier Integrity of the CACO-2 Intestinal Epithelial Cell Layer by Micronutrients
Fig 5
Effect of zinc on CACO-2 tight junctional proteins as a function of the differentiation state of the CACO-2 cell layer.
3-day, 7-day, and 21-day post-confluent CACO-2 cell layers in Falcon 75 cm2 culture flasks were refed with control medium or medium containing 100μM Zinc 48 hrs before harvesting in lysis buffer. Further steps were performed as described in Table 1. Data represents the percent of the zinc condition relative to the normalized control for each age cell layer. Data shown is expressed as the mean ± standard error for an n = 4 cell layers in all cases. NS indicates non significance. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001 (Student’s t test, two-tailed).