Remodeling of Tight Junctions and Enhancement of Barrier Integrity of the CACO-2 Intestinal Epithelial Cell Layer by Micronutrients
Fig 6
Differential effect of quercetin treatment on the tight junctional proteins of 7-day vs. 1-day post-confluent CACO-2 cell layers.
1-day and 7-day post-confluent CACO-2 cell layers in Falcon 75 cm2 culture flasks were refed with control medium or medium containing 400μM quercetin 48 hrs before harvesting in lysis buffer. Further steps were performed as described in Table 1. Data represents the percentage of band density of the no-quercetin control for that CACO-2 cell layer. Data shown is expressed as the mean ± standard error for an n = 3 cell layers in all cases. * indicates P < 0.05 for 1-day vs. 7-day changes in TJ protein (Student’s t test, two-tailed.