Remodeling of Tight Junctions and Enhancement of Barrier Integrity of the CACO-2 Intestinal Epithelial Cell Layer by Micronutrients
Fig 7
The effect of the cell layer substratum on CACO-2 epithelial barrier’s response to zinc CACO-2 cell layers were seeded at identical densities on Millicell PCF and Millicell HA units as described in Materials and Methods.
After 7 days, cell layers were refed in apical and basal-lateral compartments with control medium or medium containing 100μM zinc, 48 hrs before electrical measurements. Data represents the percentage of normalized control resistance, normalized control short circuit current, and normalized control flux rate (each relative to proper control; 2 experiments, 4 cell layers per condition). Data shown is expressed as the mean ± standard error of 8 cell layers per condition. P values (Student’s t test, two-tailed) are indicated for statistical comparisons of the various conditions.