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Molecular markers detecting circulating melanoma cells by reverse transcription polymerase chain reaction: methodological pitfalls and clinical relevance
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Adrianos Nezos
Published/Copyright:
December 5, 2008
Abstract
Herein, we expound the theory of circulating melanoma cells (CMCs) and their detection with reverse transcription polymerase chain reaction as a molecular staging approach. We discuss the molecular markers that have been used for CMC detection focusing on the use of these markers for multiplex detection analysis. Finally, we comment on the contradictory data of CMC detection studies in the literature and we propose possible solutions which may contribute to the clinical significance of CMC detection in patient management.
Clin Chem Lab Med 2009;47:1–11.
Received: 2008-5-27
Accepted: 2008-9-22
Published Online: 2008-12-05
Published Online: 2008-12-5
Published in Print: 2009-01-01
©2009 by Walter de Gruyter Berlin New York
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Keywords for this article
circulating melanoma cells;
clinical significance;
molecular markers
Articles in the same Issue
- Reviews
- Molecular markers detecting circulating melanoma cells by reverse transcription polymerase chain reaction: methodological pitfalls and clinical relevance
- HIV-GB virus C co-infection: an overview
- Genetics and Molecular Diagnostics
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- Functional polymorphisms in ACE and CYP11B2 genes and atrial fibrillation in patients with hypertensive heart disease
- No effect of MDR1 C3435T polymorphism on oral pharmacokinetics of telmisartan in 19 healthy Chinese male subjects
- A novel large deletion and three polymorphisms in the FECH gene associated with erythropoietic protoporphyria
- Aging and endothelial progenitor cell telomere length in healthy men
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