Effects of glial cell line-derived neurotrophic factor intrathecal injection on spinal dorsal horn glial fibrillary acidic protein expression in a rat model of neuropathic pain
- PMID: 22416765
- DOI: 10.3109/00207454.2012.672500
Effects of glial cell line-derived neurotrophic factor intrathecal injection on spinal dorsal horn glial fibrillary acidic protein expression in a rat model of neuropathic pain
Abstract
Glial fibrillary acidic protein (GFAP) is a specific astrocytic marker in the central nervous system. Few studies on the effects of glial cell line-derived neurotrophic factor (GDNF) intrathecal injection on GFAP expression exist in the literature. The present study determined GFAP expression in rat spinal dorsal horn following a spinal nerve ligation (SNL). The effects of GDNF intrathecal injection on GFAP expression were examined to gather experimental evidence on the mechanisms underlying neuropathic pain. Following L5-6 SNL, male Sprague-Dawley rats were randomly divided into four groups: normal control, sham-operated, SNL, and GDNF. Each group was further divided into three subgroups (n = 10) according to the times of sacrifice: 3, 7, and 14 days after surgery. Compared with the normal control and the sham-operated groups, GFAP expression in the SNL group increased at day 3 after surgery and lasted until 14 days after. GFAP expression was significantly less in the GDNF group compared with the SNL group which lasted until 14 days after surgery, suggesting that rat spinal dorsal horn GFAP expression contributes to SNL-induced neuropathic pain. The mechanisms underlying GDNF alleviation of neuropathic pain were shown to be related to the GDNF inhibition of GFAP expression in the spinal dorsal horn.
Similar articles
-
Contribution of the spinal cord BDNF to the development of neuropathic pain by activation of the NR2B-containing NMDA receptors in rats with spinal nerve ligation.Exp Neurol. 2010 Apr;222(2):256-66. doi: 10.1016/j.expneurol.2010.01.003. Epub 2010 Jan 14. Exp Neurol. 2010. PMID: 20079352
-
L6 spinal nerve ligation produces prolonged development of mechanical allodynia and gradual increase of GFAP on ipsilateral dorsal horn.Acta Neurochir (Wien). 2013 May;155(5):935-40. doi: 10.1007/s00701-012-1616-6. Epub 2013 Jan 25. Acta Neurochir (Wien). 2013. PMID: 23355062
-
Altered response to formalin by L5 spinal nerve ligation in rats: a behavioral and molecular study.Anesth Analg. 2007 Apr;104(4):936-43. doi: 10.1213/01.ane.0000258762.22607.15. Anesth Analg. 2007. PMID: 17377110
-
[Contribution of primary sensory neurons and spinal glial cells to pathomechanisms of neuropathic pain].Brain Nerve. 2008 May;60(5):483-92. Brain Nerve. 2008. PMID: 18516970 Review. Japanese.
-
Targeting the glial-derived neurotrophic factor and related molecules for controlling normal and pathologic pain.Expert Opin Ther Targets. 2016;20(2):193-208. doi: 10.1517/14728222.2016.1085972. Epub 2015 Sep 7. Expert Opin Ther Targets. 2016. PMID: 26863504 Review.
Cited by
-
Neuropathic-like Nociception and Spinal Cord Neuroinflammation Are Dependent on the TRPA1 Channel in Multiple Sclerosis Models in Mice.Cells. 2023 May 30;12(11):1511. doi: 10.3390/cells12111511. Cells. 2023. PMID: 37296632 Free PMC article.
-
Research on Association between Levels of Serum Adiponectin, Hs-CRP, and sICAM-1 and Hypertensive Cerebrovascular Complications.Biomed Res Int. 2021 Sep 13;2021:4455038. doi: 10.1155/2021/4455038. eCollection 2021. Biomed Res Int. 2021. Retraction in: Biomed Res Int. 2024 Jan 9;2024:9856308. doi: 10.1155/2024/9856308. PMID: 34557545 Free PMC article. Retracted.
-
Effects of Pulsed Radiofrequency on Nerve Repair and Expressions of GFAP and GDNF in Rats with Neuropathic Pain.Biomed Res Int. 2021 Sep 17;2021:9916978. doi: 10.1155/2021/9916978. eCollection 2021. Biomed Res Int. 2021. Retraction in: Biomed Res Int. 2024 Jan 9;2024:9802547. doi: 10.1155/2024/9802547. PMID: 34580641 Free PMC article. Retracted.
-
Differential neuropathic pain sensitivity and expression of spinal mediators in Lewis and Fischer 344 rats.BMC Neurosci. 2014 Feb 28;15:35. doi: 10.1186/1471-2202-15-35. BMC Neurosci. 2014. PMID: 24575861 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous