Immunocytochemical localization of trkA receptors in chemically identified subgroups of adult rat sensory neurons
- PMID: 7551174
- PMCID: PMC2758238
- DOI: 10.1111/j.1460-9568.1995.tb01143.x
Immunocytochemical localization of trkA receptors in chemically identified subgroups of adult rat sensory neurons
Abstract
Immunocytochemistry has been used to examine the location of trkA, the high-affinity receptor for nerve growth factor, in adult rat dorsal root ganglia, trigeminal ganglia and spinal cord. TrkA immunoreactivity was observed in small and medium sized ganglion cells and in the dorsal horn of the spinal cord. In lumbar L4 and L5 ganglia trkA-immunoreactive cells constitute 40% of dorsal root ganglion cells and range in size from 15 to 45 microns in diameter. Double labelling using markers for various dorsal root ganglion subpopulations revealed that virtually all (92%) trkA-immunoreactive cells express calcitonin gene-related peptide (CGRP) immunoreactivity. In contrast only 4 and 13% of trkA-immunoreactive cells are labelled by the monoclonal antibody LA4 or the lectin Griffonia simplicifolia IB4, markers for small non-peptide-containing cells. Eighteen percent of trkA-immunoreactive cells belong to the 'large light' subpopulation, identified by their strong immunostaining by the neurofilament antibody RT97. TrkA immunoreactivity in the dorsal horn is heaviest in laminae I and II outer, has a similar distribution to CGRP, and is depleted by dorsal rhizotomy. Our results show that trkA-expressing cells in dorsal root ganglia correspond almost exactly with the CGRP, peptide-producing population. The receptor is present not only on cell bodies but also on central terminals. Non-peptide-containing small cells, which constitute 30% of dorsal root ganglion cells, are not trkA-immunoreactive and therefore most probably are functionally independent of nerve growth factor.
Figures








Similar articles
-
Nerve growth factor treatment increases brain-derived neurotrophic factor selectively in TrkA-expressing dorsal root ganglion cells and in their central terminations within the spinal cord.J Neurosci. 1997 Nov 1;17(21):8476-90. doi: 10.1523/JNEUROSCI.17-21-08476.1997. J Neurosci. 1997. PMID: 9334420 Free PMC article.
-
TrkA immunoreactive neurones in the rat spinal cord.J Comp Neurol. 1997 Sep 1;385(3):441-55. J Comp Neurol. 1997. PMID: 9300770
-
Differential expression of the mRNA for the vanilloid receptor subtype 1 in cells of the adult rat dorsal root and nodose ganglia and its downregulation by axotomy.J Neurosci. 1999 Mar 1;19(5):1844-54. doi: 10.1523/JNEUROSCI.19-05-01844.1999. J Neurosci. 1999. PMID: 10024368 Free PMC article.
-
Presence or absence of TrkA protein distinguishes subsets of small sensory neurons with unique cytochemical characteristics and dorsal horn projections.J Comp Neurol. 1995 Oct 23;361(3):404-16. doi: 10.1002/cne.903610305. J Comp Neurol. 1995. PMID: 8550888
-
Co-localization of endomorphin-2 and substance P in primary afferent nociceptors and effects of injury: a light and electron microscopic study in the rat.Eur J Neurosci. 2004 Apr;19(7):1789-99. doi: 10.1111/j.1460-9568.2004.03284.x. Eur J Neurosci. 2004. PMID: 15078552
Cited by
-
KV4 channels in isolectin B4 muscle dorsal root ganglion neurons of rats with experimental peripheral artery disease: effects of bradykinin B1 and B2 receptors.Am J Physiol Regul Integr Comp Physiol. 2022 Nov 1;323(5):R616-R627. doi: 10.1152/ajpregu.00117.2022. Epub 2022 Sep 12. Am J Physiol Regul Integr Comp Physiol. 2022. PMID: 36094447 Free PMC article.
-
Activation of extracellular signal-regulated protein kinase 5 is essential for cystitis- and nerve growth factor-induced calcitonin gene-related peptide expression in sensory neurons.Mol Pain. 2012 Jun 28;8:48. doi: 10.1186/1744-8069-8-48. Mol Pain. 2012. PMID: 22742729 Free PMC article.
-
NGF - the TrkA to successful pain treatment.J Pain Res. 2012;5:279-87. doi: 10.2147/JPR.S33408. Epub 2012 Aug 17. J Pain Res. 2012. PMID: 23028238 Free PMC article.
-
Dynamic pattern of reg-2 expression in rat sensory neurons after peripheral nerve injury.J Neurosci. 2002 Sep 1;22(17):7493-501. doi: 10.1523/JNEUROSCI.22-17-07493.2002. J Neurosci. 2002. PMID: 12196572 Free PMC article.
-
An automated method for the quantification of transgene expression in motor axons of the peripheral nerve.J Neurosci Methods. 2018 Oct 1;308:346-353. doi: 10.1016/j.jneumeth.2018.09.005. Epub 2018 Sep 5. J Neurosci Methods. 2018. PMID: 30194042 Free PMC article.
References
-
- Alvarez FJ, Rodrigo J, Jessell TM, Dodd J, Priestley JV. Ultrastructure of primary afferent fibres and terminals expressing alpha-galactose extended oligosaccharides in the spinal cord and brainstem of the rat. J Neurocytol. 1989;18:631–645. - PubMed
-
- Alvarez FJ, Moms HR, Priestley JV. Sub-populations of smaller diameter trigeminal primary afferent neurons defined by expression of calcitonin gene-related peptide and the cell surface oligosaccharide recognized by monoclonal antibody LA4. J Neurocytol. 1991;20:716–731. - PubMed
-
- Ambalavanar R, Morris R. The distribution of binding by isolectin I-B4 from Griflonia simplicifolia in the trigeminal ganglion and brainstem trigeminal nuclei in the rat. Neuroscience. 1992;47:421–429. - PubMed
-
- Andreev N, Inuishin M, McMahon SB. Nerve growth factor acutely enhances the responsiveness of dorsal horn neurones to noxious heat. Soc Neurosci Abstr. 1994;20:455.16.
-
- Arvidsson U, Risling M, Frisen J, Piehl F, Fried K, Hökfelt T, Cullheim S. TrkC-like immunoreactivity in the primate descending serotoninergic system. Eur J Neurosci. 1994;6:230–236. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials